|
KMID : 0364820200560020160
|
|
Korean Journal of Microbiology
2020 Volume.56 No. 2 p.160 ~ p.169
|
|
|
Isolation of Lactobacillus from donkey dung and its probiotic characterization
|
|
Kathade Suyash Arunrao
Aswani Mayur Arjun
Anand Pashmin Kaur
Jagtap Suresh
Bipinraj Niricharan Kunchiraman
|
|
|
Abstract
|
|
|
|
Probiotics are live microorganisms which upon ingestion confer health benefits to the host such as prevention of infection in the digestive tract, activate, and modulate the immune response and increase the number of native bacteria in the gut. The present study was aimed to isolate bacteria from donkey dung and characterize for probiotic activity. Bacterial cultures were isolated from excreta of infant donkey and were characterized using standard procedures. Cultures were grown anaerobically, and in total 16 cultures showing Lactobacillus morphology were further screened for the probiotic property. Isolate LB-VII was found to be non-hemolytic and has the ability to tolerate 1.2% bile, pH 1.5~10, 8% NaCl as well as showed growth at 42°C. The culture survived gastric and intestinal environment and showed bile salt hydrolysis activity. LB-VII exhibited 100% auto-aggregation and hydrophobic reaction. The culture could also co-aggregate with Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus, a property, which is required to control pathogens. Moreover, the isolate resist a wide range of antibiotic. All these characters make LB-VII a good probiotic culture and was identified as L. plantarum by molecular methods.
|
|
|
KEYWORD
|
|
|
Lactobacillus, donkey dung, probiotics
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|